| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075640 |
| Chromosome: | chromosome 12 |
| Location: | 3036784 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500700 | LCL2 | (1 of 125) IPR001841 - Zinc finger, RING-type; Long-chain-fatty-acid-CoA ligase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGAGGGAAGGAAACACGCAAGACTTGT |
| Internal bar code: | GCCTGAGTCGTCATCTTGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.4063 |
| LEAP-Seq n confirming: | 1172 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCGTTTATGCTCGATTG |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |