Insertion junction: LMJ.RY0402.075652_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075652
Chromosome:chromosome_17
Location:3323971
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g723300 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):GCTTGCCTTGCAGGCATGAGGGTGATTTCA
Internal bar code:CACGGGGCATGTGGAGGGGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:642
LEAP-Seq percent confirming:99.7696
LEAP-Seq n confirming:866
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGTTGCAGGATTTGTGGG
Suggested primer 2:ATATGATGGCGTCTTCCTCG