| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075661 |
| Chromosome: | chromosome 10 |
| Location: | 4941763 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455000 | (1 of 1) IPR010995//IPR011009 - DNA repair Rad51/transcription factor NusA, alpha-helical // Protein kinase-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGACACGGAGCCGTGGACATGCGCCAC |
| Internal bar code: | GGAACGTGGGAAAGCTGGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 229 |
| LEAP-Seq percent confirming: | 32.478 |
| LEAP-Seq n confirming: | 2987 |
| LEAP-Seq n nonconfirming: | 6210 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCTCGCAAATATGTAGA |
| Suggested primer 2: | CGTGCAAACCACAAATAACG |