Insertion junction: LMJ.RY0402.075663_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075663
Chromosome:chromosome 17
Location:3956010
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g728650 FAP196 (1 of 2) PTHR13720//PTHR13720:SF14 - WD-40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 16; Flagellar Associated Protein 196 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCAGACACCGACACCGACACCGACACTG
Internal bar code:CAACCCCATTTGCTCCGACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:241
LEAP-Seq percent confirming:24.0759
LEAP-Seq n confirming:749
LEAP-Seq n nonconfirming:2362
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTGAAGGACGATGGAGGG
Suggested primer 2:CCTCACCCACACAAACACAC