| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075666 |
| Chromosome: | chromosome 6 |
| Location: | 7306095 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298750 | AOT4 | (1 of 9) PF01490 - Transmembrane amino acid transporter protein (Aa_trans); Amino acid transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGTGTATCGTGAATGGCACTTCTCCCT |
| Internal bar code: | GGTGGTGTTCGCTCGGCAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 341 |
| LEAP-Seq percent confirming: | 99.2991 |
| LEAP-Seq n confirming: | 850 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACTTCTCCACGGTGTTC |
| Suggested primer 2: | TGTCTCAGCAGGAAGGTGTG |