| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075668 |
| Chromosome: | chromosome_16 |
| Location: | 147323 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g695200 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCTTTCCCTTGCCTTTGCCCTCACTCAC |
| Internal bar code: | AGCGAAGGGGCAAACGACGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 85.7424 |
| LEAP-Seq n confirming: | 872 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTACCAAGGCAGTTGTG |
| Suggested primer 2: | GACACTTGCCCCTACACGAT |