Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.075686 |
Chromosome: | chromosome_13 |
Location: | 3733037 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589350 | FBB6 | Flagellar/basal body protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTTCTTGGGCTTTCACGTTGCACCGTGGA |
Internal bar code: | TTAGAAAGCCCGTTGTCAACGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 142 |
LEAP-Seq percent confirming: | 99.8547 |
LEAP-Seq n confirming: | 1374 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGAGCTAACCACCTCCATCA |
Suggested primer 2: | CGCGAGGTGAGTTTAGTTCC |