Insertion junction: LMJ.RY0402.075686_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.075686
Chromosome:chromosome 13
Location:3733037
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTCTTGGGCTTTCACGTTGCACCGTGGA
Internal bar code:TTAGAAAGCCCGTTGTCAACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:142
LEAP-Seq percent confirming:99.8547
LEAP-Seq n confirming:1374
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGCTAACCACCTCCATCA
Suggested primer 2:CGCGAGGTGAGTTTAGTTCC