Insertion junction: LMJ.RY0402.075686_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.075686
Chromosome:chromosome 13
Location:3733038
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTATCGTAATCATGGTGCGTGGCACTCA
Internal bar code:ACATCAGAGGGGCAAAAACCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:98.5352
LEAP-Seq n confirming:1009
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGCTAACCACCTCCATCA
Suggested primer 2:CGCGAGGTGAGTTTAGTTCC