Insertion junction: LMJ.RY0402.075692_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.075692
Chromosome:chromosome 6
Location:3910675
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g278183 (1 of 1) PTHR23054//PTHR23054:SF14 - UNCHARACTERIZED // PROTEIN Y45F10A.7, ISOFORM A 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCTCTCGCCACCTGTCGTCGCGCGCGCA
Internal bar code:GACGGGCAACGGAAACGTCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:318
LEAP-Seq percent confirming:98.4211
LEAP-Seq n confirming:561
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAAAAAGCTTATCGACCA
Suggested primer 2:ATATGTCCAAACCATCCCGA