| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075728 |
| Chromosome: | chromosome 16 |
| Location: | 5488073 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680750 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGTGTCCCCTCATGATTTTTGAGTGCT |
| Internal bar code: | TGGCGGTAGGAACGGAAAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 87.4092 |
| LEAP-Seq n confirming: | 361 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCTTAGTGCGTGCTGGT |
| Suggested primer 2: | CAGAACGTACCTCGCTGTGA |