| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075733 |
| Chromosome: | chromosome 13 |
| Location: | 3493129 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g587800 | NUP188 | (1 of 1) PTHR31431:SF1 - NUCLEOPORIN NUP188 HOMOLOG; Nucleoporin 188 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGGCAGCGAGTGCGGAATGGCGCCAAC |
| Internal bar code: | GTATGTGGCGGCCGGTTCTGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 99.656 |
| LEAP-Seq n confirming: | 2897 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTCGAAGATGTCGTTTG |
| Suggested primer 2: | GACAGCAGGTCCTCCAGAAG |