Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.075745 |
Chromosome: | chromosome 11 |
Location: | 921515 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g467659 | (1 of 1) K15128 - mediator of RNA polymerase II transcription subunit 6 (MED6) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTTTGGCCTGGCCGCTTTCTTACCCCT |
Internal bar code: | AGTGTTGGTCATGAGCGGCTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1 |
LEAP-Seq percent confirming: | 99.1617 |
LEAP-Seq n confirming: | 4968 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGAGTGAAGCGCTGGTCTT |
Suggested primer 2: | GGGAGGGAGAGTGTGTGTGT |