| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075765 |
| Chromosome: | chromosome 9 |
| Location: | 1700245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396900 | (1 of 1) K03426 - NAD+ diphosphatase (E3.6.1.22, NUDT12, nudC); Nudix phosphohydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCATAGTGATTCCGCTCGTCCGTCTCTTG |
| Internal bar code: | GAAACGGGTATGGAATCCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 99.7183 |
| LEAP-Seq n confirming: | 354 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGCAGTGGTACGACAAG |
| Suggested primer 2: | AGCTACTCCCGGTCCTGTTT |