| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075786 |
| Chromosome: | chromosome 12 |
| Location: | 1654512 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486209 | (1 of 1) K09533 - DnaJ homolog subfamily C member 13 (DNAJC13) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCCGGGCGCATGTTTGTGCGTATGTG |
| Internal bar code: | ATCATGCGCGGTCTGTACAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 99.3878 |
| LEAP-Seq n confirming: | 2760 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGGAGTGCAGTGTTGTCG |
| Suggested primer 2: | CGTTTCTCGTGTTTTCCCAT |