| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075789 |
| Chromosome: | chromosome 2 |
| Location: | 409544 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076050 | (1 of 2) IPR000008//IPR000104 - C2 domain // Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTGTGAATTCGCGGAGCACACAGATGAG |
| Internal bar code: | TAAGATTCGTGGCAACGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 270 |
| LEAP-Seq percent confirming: | 99.0123 |
| LEAP-Seq n confirming: | 401 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGTTGGGTTCGTGAAGT |
| Suggested primer 2: | TTGTCCATAACCTGCACAGC |