| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075792 |
| Chromosome: | chromosome 3 |
| Location: | 3748428 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169850 | TSC1 | (1 of 1) PTHR10890:SF3 - CYSTEINE--TRNA LIGASE, CYTOPLASMIC; Cysteinyl-tRNA synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGAGGCGTTCCGGCTTGCTCCACCGCGT |
| Internal bar code: | TGCCCGAGGCGGCATGACACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 51.9481 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCACGCATTCTTTTGCTC |
| Suggested primer 2: | CGCTCAAAAACTTCGTCACA |