Insertion junction: LMJ.RY0402.075794_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.075794
Chromosome:chromosome 2
Location:2724934
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g093700 (1 of 1) K14951 - cation-transporting ATPase 13A3/4/5 (ATP13A3_4_5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGACCATTGCCAGCATGATTACTGTGG
Internal bar code:CATCCGATCAAATTGCCAATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:287
LEAP-Seq percent confirming:99.249
LEAP-Seq n confirming:1718
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAGGTTGGTCTCAAACGC
Suggested primer 2:GACCTAATGCTTCTGCTGGG