| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075808 |
| Chromosome: | chromosome 5 |
| Location: | 3388374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241000 | (1 of 2) PF10084 - Uncharacterized protein conserved in bacteria (DUF2322) (DUF2322) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGTTCGCTCCCTTGTCAACCTAGGCC |
| Internal bar code: | CGACGGAGTCTCGGGCACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 94.8577 |
| LEAP-Seq n confirming: | 2933 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAAGGAAGCAGAGGAAAA |
| Suggested primer 2: | TGCAAACGCATCTAGTGGAG |