Insertion junction: LMJ.RY0402.075824_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075824
Chromosome:chromosome 8
Location:4925439
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g385150 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTACTAGTCAGAACCCAGCTCGTGCGCCC
Internal bar code:GGGATAGCGGGCGAAAGCCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:87.2399
LEAP-Seq n confirming:1593
LEAP-Seq n nonconfirming:233
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AACAGTCAATGGGTTCTCGC
Suggested primer 2:GTCTGCATCCACCTTCACAG