| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075838 |
| Chromosome: | chromosome 14 |
| Location: | 856427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613350 | (1 of 1) K14016 - ubiquitin fusion degradation protein 1 (UFD1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGCGACCTCACTTCCTGTCAGCAATAAA |
| Internal bar code: | GGGACCTCCCTGAGGGTCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 149 |
| LEAP-Seq percent confirming: | 99.5357 |
| LEAP-Seq n confirming: | 2144 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAGCACGTATCTTCTTG |
| Suggested primer 2: | TGACGAGCACTATCACTCCG |