| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075838 |
| Chromosome: | chromosome 17 |
| Location: | 53610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696550 | uS8m,MRPS8 | Mitochondrial ribosomal protein S8 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGGGCAGGCAAGGCCACGTGGAGGAGC |
| Internal bar code: | AACGCGGTGACTTCTCCAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 96.2402 |
| LEAP-Seq n confirming: | 1971 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGCAGACGGAGGATTGCT |
| Suggested primer 2: | GATACGGCAGCCTTCTCAAG |