| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075852 |
| Chromosome: | chromosome 16 |
| Location: | 1169524 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650550 | FAP103 | Flagellar Associated Protein 103; (1 of 4) K00940 - nucleoside-diphosphate kinase (ndk, NME) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTGCTTCGGCGTGCGGAGACGCCGCGT |
| Internal bar code: | GGCTTGGCAGGCGTGGTGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 680 |
| LEAP-Seq percent confirming: | 98.6498 |
| LEAP-Seq n confirming: | 3580 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTGTGCAACTATCAGGA |
| Suggested primer 2: | TGGTTACGAAAGATGGCTCC |