| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075863 |
| Chromosome: | chromosome 17 |
| Location: | 3451373 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724700 | PAO3,Tic55-like3,TIC55-3,PAO1 | Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTGTTGCATACCACCACAAGTAACCC |
| Internal bar code: | TATACCGGCAGAGGGCTCGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 95.7173 |
| LEAP-Seq n confirming: | 2302 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGTTCATGAGCAATCTCG |
| Suggested primer 2: | GCAAGTGGTGATGCAGAAGA |