| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075866 |
| Chromosome: | chromosome 12 |
| Location: | 2398682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507250 | (1 of 1) K18681 - DIS3-like exonuclease 1 (DIS3L) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTATGCTTCCTGGTGAGTGGTAACAC |
| Internal bar code: | TTGAGCGGAGTTCGGTTCCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 404 |
| LEAP-Seq percent confirming: | 98.0594 |
| LEAP-Seq n confirming: | 6013 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTGTCAGCCTCCACAT |
| Suggested primer 2: | TCGCTCATGAAATCTACCCC |