Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.075872 |
Chromosome: | chromosome 2 |
Location: | 905310 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g079450 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACACACAGCAGAAACCTGCGGCACTGGT |
Internal bar code: | AACCGCGGACTGTTTAAGTCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 677 |
LEAP-Seq percent confirming: | 98.1454 |
LEAP-Seq n confirming: | 3334 |
LEAP-Seq n nonconfirming: | 63 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGTACTTAGTGCGGCCCAT |
Suggested primer 2: | GCAGCTAAATAGGTCGGCAG |