Insertion junction: LMJ.RY0402.075886_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.075886
Chromosome:chromosome 9
Location:6026743
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g403108 (1 of 1) K16601 - tubulin polyglutamylase TTLL4 [EC:6.-.-.-] (TTLL4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGGTTTGCCTGGGTTGCGCAAGACGAAT
Internal bar code:TTGCGATAAACATCTCTTGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:589
LEAP-Seq percent confirming:98.9011
LEAP-Seq n confirming:360
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTCCCCAAATGAGTTAT
Suggested primer 2:AAGTACTACGCCCAACGCAT