| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075904 |
| Chromosome: | chromosome 3 |
| Location: | 8250815 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199500 | FAP129 | Flagellar Associated Protein 129 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGAGAATTGAAGTCTACGCCCGCCGGT |
| Internal bar code: | ACAGGCGCAATTAACTTCTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 644 |
| LEAP-Seq percent confirming: | 99.4217 |
| LEAP-Seq n confirming: | 2407 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGTCTCGCCTAAAAGCCA |
| Suggested primer 2: | ACAGAAGGCGTTGAGAGCAT |