| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.075910 |
| Chromosome: | chromosome_17 |
| Location: | 1044160 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g703600 | OFD1 | Basal body protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGTCAGCGCAATTTCGGAGGCGGCGGCG |
| Internal bar code: | GGATCGTTTCAGTTGGAAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 418 |
| LEAP-Seq percent confirming: | 99.4186 |
| LEAP-Seq n confirming: | 1197 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCATCATCTCCTCCTCC |
| Suggested primer 2: | CAGCAACTGCGTCTTGAGAG |