Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.075912 |
Chromosome: | chromosome_17 |
Location: | 4491326 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g732500 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCCTGTCTGATCGCCCCACCTCATGAACCA |
Internal bar code: | CCGGCGGCGAGGCAAGCGTTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 629 |
LEAP-Seq percent confirming: | 98.4655 |
LEAP-Seq n confirming: | 2695 |
LEAP-Seq n nonconfirming: | 42 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCATGGGTTTGTGATCTGT |
Suggested primer 2: | GTTCAAGACATGCAGGAGCA |