| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.075934 |
| Chromosome: | chromosome 16 |
| Location: | 5368835 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681600 | CSV14 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCATGCATGGTTTGCGCCACGGGTTTGC |
| Internal bar code: | GGTGTGCTGTAGGCGGCTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 99.3289 |
| LEAP-Seq n confirming: | 888 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGACTGGTCATCTCAGCA |
| Suggested primer 2: | CCGAGTTCAGCGTATTTGGT |