Insertion junction: LMJ.RY0402.075938_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.075938
Chromosome:chromosome 6
Location:2181805
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g265950 DHC3 Axonemal Inner Arm Dynein Heavy Chain 3, pseudogene; (1 of 2) PTHR10676:SF136 - DYNEIN HEAVY CHAIN 6, AXONEMAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGGAAACAACAACTCCCCCTCCAGGTTC
Internal bar code:CCACGCGGGCACACCCCCGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:1078
LEAP-Seq percent confirming:96.356
LEAP-Seq n confirming:12005
LEAP-Seq n nonconfirming:454
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTGCGATACGATTACGA
Suggested primer 2:GGTATCGATGCGGTTGAACT