Insertion junction: LMJ.RY0402.075965_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.075965
Chromosome:chromosome 10
Location:1904479
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g431900 (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGGACCCTGAAATTCGACGACCGACCC
Internal bar code:CTTCCAGGTGTGGTGACGCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:967
LEAP-Seq percent confirming:99.6983
LEAP-Seq n confirming:5288
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAGCCGCACTTAAAATC
Suggested primer 2:GTACTCAACCAGGATGGCGT