Insertion junction: LMJ.RY0402.075973_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.075973
Chromosome:chromosome 6
Location:4087904
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278216 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGGGGTGAAACACCACTCGGTTCACCCC
Internal bar code:TTATACCTGATCAATATTCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:553
LEAP-Seq percent confirming:98.9645
LEAP-Seq n confirming:8315
LEAP-Seq n nonconfirming:87
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGAATGTGTTTGTGCTG
Suggested primer 2:CTATTGGAGTGGTGGGCTGT