Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.075982 |
Chromosome: | chromosome 10 |
Location: | 915356 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g424350 | (1 of 1) PTHR24209:SF7 - PROTEIN DA1-RELATED 2 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTAGGCGGAGCGGGGGCTATGGCTGCC |
Internal bar code: | GACTTTTATTCTTGAACACGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 732 |
LEAP-Seq percent confirming: | 99.4948 |
LEAP-Seq n confirming: | 2757 |
LEAP-Seq n nonconfirming: | 14 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGTGTGTGGGTTTCTTTG |
Suggested primer 2: | TGAGAACTCGACAAAATGCG |