| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.075988 |
| Chromosome: | chromosome 12 |
| Location: | 8340593 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549500 | (1 of 2) 1.5.4.1 - Pyrimidodiazepine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCCGAGGGTGGCGCGCTCTTTGGCTG |
| Internal bar code: | GCGCTGGCGGTACACCGTTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 98.2955 |
| LEAP-Seq n confirming: | 1730 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACGCTGCCTCTAAGTTGG |
| Suggested primer 2: | GGGGACAGGCGTAAGTACAA |