| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.075990 |
| Chromosome: | chromosome 4 |
| Location: | 386722 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217915 | NAR1C | Formate/nitrite transporter; (1 of 1) IPR000292//IPR023271 - Formate/nitrite transporter // Aquaporin-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTAGCTCCAAGACAAAAACGGCCGGGT |
| Internal bar code: | TTGGCAGGGCTTCCTTTTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 99.6546 |
| LEAP-Seq n confirming: | 577 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCCCCTCCTATGGTATT |
| Suggested primer 2: | GCTCCTCCTCCTCCTCAACT |