| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076016 |
| Chromosome: | chromosome 3 |
| Location: | 7611799 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204500 | CSE17 | Precursor of chemotactic amidated peptide | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGAGCGTCGTGCCCGGCACACTCAGT |
| Internal bar code: | TGAACAGGGCCCTCCGATTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 547 |
| LEAP-Seq percent confirming: | 99.115 |
| LEAP-Seq n confirming: | 2912 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTGAGTAACCCCTGCG |
| Suggested primer 2: | GCCTGCCTTGTGGTGTAGAT |