| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076018 |
| Chromosome: | chromosome 9 |
| Location: | 1788734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396250 | VTE5 | Phosphatidate cytidylyltransferase; (1 of 1) K18678 - phytol kinase (VTE5) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGTGTGGTGTGGTGCGGGGGCTGAGGGC |
| Internal bar code: | GGTAACTAAACCTTTCGCACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 669 |
| LEAP-Seq percent confirming: | 98.761 |
| LEAP-Seq n confirming: | 3029 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGTCCCTGCCCATCAAC |
| Suggested primer 2: | CGGTACTGCCCGTAACTTGT |