Insertion junction: LMJ.RY0402.076048_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.076048
Chromosome:chromosome 16
Location:3647101
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g688450 CFAP298,DAB2,C21ORF59,FBB18 (1 of 1) PF11069 - Protein of unknown function (DUF2870) (DUF2870); Flagellar basal body proteome 18 / Dynein assembly factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCCCAGCCCTCAGAATCGTTCTTGAACT
Internal bar code:CGGAGTGCTCATCTCCGACTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1064
LEAP-Seq percent confirming:67.9884
LEAP-Seq n confirming:2109
LEAP-Seq n nonconfirming:993
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GCTAGCAGAGGACGAGGATG
Suggested primer 2:AGAGGGTGTATTGGGTGCTG