| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076055 |
| Chromosome: | chromosome 8 |
| Location: | 2317132 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370500 | (1 of 1) IPR013024//IPR017939 - Butirosin biosynthesis, BtrG-like // Gamma-glutamylcyclotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGGGTTGCGAGGTGTAAGCAGTGGGAA |
| Internal bar code: | TCTTGCGGCGCCCGTGTAACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 136 |
| LEAP-Seq percent confirming: | 53.8471 |
| LEAP-Seq n confirming: | 13710 |
| LEAP-Seq n nonconfirming: | 11751 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCACTCCGTGCTTTATGA |
| Suggested primer 2: | CAAAATGAAGTCGTCGAGCA |