Insertion junction: LMJ.RY0402.076059_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.076059
Chromosome:chromosome 6
Location:7699790
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g301850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATAACTAGGTCGACTGCCATGTTGCAGTA
Internal bar code:AGCAACGACTATCCCCAAATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:523
LEAP-Seq percent confirming:96.809
LEAP-Seq n confirming:6644
LEAP-Seq n nonconfirming:219
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGAATCTGTGCTGGTTA
Suggested primer 2:TGGTTTCCTCCATGGTCTTC