Insertion junction: LMJ.RY0402.076062_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.076062
Chromosome:chromosome 1
Location:3252539
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g020400 (1 of 1) K13111 - WD40 repeat-containing protein SMU1 (SMU1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCGCCACCTCTTACCTCCAACCCACCC
Internal bar code:TCTCCTGCGTATGGCGCGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:466
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:398
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGGTGGCTACATCAGG
Suggested primer 2:TCCACCTCCCTACACCTTTG