| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.076063 |
| Chromosome: | chromosome 2 |
| Location: | 3415931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095110 | FAP255 | Flagellar Associated Protein 255; (1 of 1) K17553 - protein phosphatase 1 regulatory subunit 11 (PPP1R11) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTATCTGACATTGCACATATTGCTCGGG |
| Internal bar code: | GCGACGTGCGTTTCCGGCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 98.2973 |
| LEAP-Seq n confirming: | 4272 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCATGCCTCTCAATGTGC |
| Suggested primer 2: | AGCTGCCCTGACGCTTATTA |