Insertion junction: LMJ.RY0402.076089_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.076089
Chromosome:chromosome 2
Location:3532357
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095126 DPE1,DPE2 Disproportionating enzyme 2; (1 of 2) 2.4.1.25 - 4-alpha-glucanotransferase / Oligo-1,4-1,4-glucantransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAGTAATCGTGAATGTGACTCCCCCCCTC
Internal bar code:ACGCCGAACTGCCGTGACTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:330
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCACGGGAACTGGATTAT
Suggested primer 2:TAGAGGGGAAGAAAGGGGAA