| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076105 |
| Chromosome: | chromosome 10 |
| Location: | 1906901 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431950 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCACTGTTGATGAAACTAGGAATGGACT |
| Internal bar code: | TCCCTAAGGTAGAATATTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1026 |
| LEAP-Seq percent confirming: | 99.1317 |
| LEAP-Seq n confirming: | 685 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTACTGACGTCCGAGTT |
| Suggested primer 2: | ATTATACCGTGCGCAAGACC |