Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.076106 |
Chromosome: | chromosome_1 |
Location: | 5111325 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g035500 | VPS34 | Phosphatidylinositol-3-kinase, probably vacuolar | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTCACCGCAAATGGTGAAACACACACCCC |
Internal bar code: | TATTCAGCGTCAGTCACAATTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 84 |
LEAP-Seq percent confirming: | 66.8306 |
LEAP-Seq n confirming: | 1902 |
LEAP-Seq n nonconfirming: | 944 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTGCAAGGAGATGATCGAG |
Suggested primer 2: | CATCTTACGTCACCCGACCT |