| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.076119 |
| Chromosome: | chromosome 9 |
| Location: | 1797061 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396200 | CSV4 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATAACAATCCACGGAAGTCTCAACTCAG |
| Internal bar code: | TGTTGATCGACCATGGGTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 892 |
| LEAP-Seq percent confirming: | 99.7009 |
| LEAP-Seq n confirming: | 18002 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGACTCATCCAGAACCA |
| Suggested primer 2: | AAAGCTGAAAGGGAAGGAGC |