| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.076209 |
| Chromosome: | chromosome 11 |
| Location: | 779224 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467635 | MOT22 | Predicted protein; (1 of 1) PF00339 - Arrestin (or S-antigen), N-terminal domain (Arrestin_N) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTTCATTATTTCAGGGAACGGATGCAT |
| Internal bar code: | GTGGTACGGGTGCTCTCGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 99.1119 |
| LEAP-Seq n confirming: | 1674 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATACGGCACGCTCTGTTG |
| Suggested primer 2: | CGAACAGGTGCTGCTTCATA |