Insertion junction: LMJ.RY0402.076224_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.076224
Chromosome:chromosome 5
Location:177101
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAGACGTGATGCTCGTCCTCCACATCATC
Internal bar code:CGTACGCTGCCGGACGACCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:652
LEAP-Seq percent confirming:99.2267
LEAP-Seq n confirming:2053
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTATGGCACAACAATGC
Suggested primer 2:AAGACAGTGTGCCTGCTGTG