| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.076241 |
| Chromosome: | chromosome 1 |
| Location: | 3481495 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022100 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGAACACGAGGTGCTCCGGGTCCTCGC |
| Internal bar code: | CGTGCCGACGTACAGGGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 656 |
| LEAP-Seq percent confirming: | 99.4012 |
| LEAP-Seq n confirming: | 2324 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGGCAGAAGACACACC |
| Suggested primer 2: | GGCAGGATAGGTGACATCGT |